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- Download the protein sequences hypoxanthine
phosphoribosyl transferase (HPRT) for mouse and E. coli in
FASTA format from the NCBI database.
- Perform a dot matrix alignment using the program Dothelix
http://www.genebee.msu.su/services/dhm/advanced.html.
- Perform pairwise alignment the program ClustalW2
http://www.ebi.ac.uk/Tools/clustalw2/
- Perform a local alignment using the program LALIGN
http://www.ch.embnet.org/software/LALIGN_form.html.
- Perform a global alignment using the program LALIGN (Compare
the global and local alignment).
- Change the gap penalty from "-14/-4" to "-4/-1" and run a local
alignment. (Compare with previous results).
- Do a pairwise alignment using BLAST (bl2seq
program located under section "Specialized BLAST" on the
NCBI BLAST homepage).
(Compare with previous results).
- Use the exhaustive alignment program SSEARCH
http://pir.georgetown.edu/pirwww/search/pairwise.shtml (Compare
with previous results).
- Finally check with the program PRSS
http://fasta.bioch.virginia.edu/fasta_www2/fasta_www.cgi?rm=shuffle if there is any statistically significant similarity between the two
sequences. (Study the output and try to find the critical statistical
parameters).
Christoph Flamm
2009-01-06