RNAplex − manual page for RNAplex 2.4.18
Find targets of a query RNA
reads two RNA
sequences from stdin or <filename> and computes
optimal and suboptimal secondary structures for their
hybridization. The calculation is simplified by allowing
only inter−molecular base pairs. Accessibility effects
can be estimated by RNAplex if a RNAplfold accessibility
profile is provided. The computed optimal and suboptimal
structure are written to stdout, one structure per line.
Each line consist of: The structure in dot bracket format
with a "&" separating the two strands. The
range of the structure in the two sequences in the format
"from,to : from,to"; the energy of duplex
structure in kcal/mol. The format is especially useful for
computing the hybrid structure between a small probe
sequence and a long target sequence.
Print help and exit
Print help, including all details and hidden options, and exit
Print version and exit
Below are command line options which alter the general input behavior of this program
File containing the query sequence.
Input sequences can be given piped to RNAplex or given in a query file with the −q option. Note that the −q option implies that the −t option is also used
File containing the target sequence.
Input sequences can be given piped to RNAplex or given in a target file with the −t option. Note that the −t option implies that the −q option is also used
Location of the accessibility profiles.
This option switches the accessibility modes on and indicates in which directory accessibility profiles as generated by RNAplfold can be found
Allow the reading and parsing of memory dumped opening energy file
The −b option allows one to read and process opening energy files which are saved in binary format
This can reduce by a factor of 500x−1000x the time needed to process those
files. RNAplex recognizes the corresponding opening energy files by looking for files named after the sequence and containing the suffix _openen_bin. Please look at the man page of RNAplfold if you need more information on how to produce binary opening energy files.
Read energy parameters from paramfile, instead of using the default parameter set.
Different sets of energy parameters for RNA and DNA should accompany your distribution. See the RNAlib documentation for details on the file format. When passing the placeholder file name "DNA", DNA parameters are loaded without the need to actually specify any input file.
Options which alter the computing behaviour of RNAplex.
Rescale energy parameters to a temperature T. Default is 37C.
Maximal length of an interaction (default=‘40’)
Maximal allowed length of an interaction
Cost to add to each nucleotide in a duplex (default=‘0’)
Cost of extending a duplex by one nucleotide. Allows one to find compact duplexes, having few/small bulges or interior loops Only useful when no accessibility profiles are available. This option is disabled if accessibility profiles are used (−a option)
Compute Tm for probes (default=off)
Use this option if you want to compute the melting temperature of your probes
Set the probe concentration for the Tm
−N, −−na−concentration=DOUBLE Set the Na+ concentration for the Tm
−M, −−mg−concentration=DOUBLE Set the Mg2+ concentration for the Tm
Set the K+ concentration for the Tm computation
Set the tris+ concentration for the Tm
Speedup of the target search (default=‘0’)
This option allows one to decide if the backtracking has to be done (−f 0, −f 2) or not (−f 1). For −f 0 the structure is computed based on the standard energy model. This is the slowest and most precise mode of RNAplex. With −f 2, the structure is computed based on the approximated plex model. If a lot of targets are returned this is can greatly improve the runtime of RNAplex. −f 1 is the fastest mode, as no structure are recomputed
Rescale all opening energy by a factor V
Scale−factor for the accessibility. If V is set to 1 then the scaling has no effect on the accessibility.
Calculate structures subject to constraints. (default=off)
The program reads first the sequence, then a string containing constraints on the structure for the query sequence encoded with the symbols: . (no constraint for this base) | (the corresponding base has to be paired)
Tells RNAplex to compute interactions based on alignments
If the A option is set RNAplex expects clustalw files as input for the −q and −t option.
If set, RNAplex will convert all opening energy file in a directory set by the −a option into binary opening energy files
RNAplex can be used to convert existing text formatted opening energy files into binary formatted files. In this mode RNAplex does not compute interactions.
Options that modify the output
Distance between target 3’ ends of two consecutive duplexes
Distance between the target 3’ends of two consecutive duplexes. Should be set to the maximal length of interaction to get good results
Smaller z leads to larger overlaps between consecutive duplexes.
Minimal energy for a duplex to be
Energy threshold for a duplex to be returned. The threshold is set on the total energy of interaction, i.e. the hybridization energy corrected for opening energy if −a is set or the energy corrected by −c. If unset, only the mfe will be returned
Draw an alignment annotated interaction from RNAplex
This option allows one to produce interaction figures in PS−format a la RNAalifold, where base−pair conservation is represented in color−coded format. In this mode no interaction are computed, but the −I option indicates the location of the file containing interactions between two RNA (alignments/sequence) from a previous run. If the −A option is not set a structure figure a la RNAfold with color−coded annotation of the accessibilities is returned
Tells how large the region around the target site should be for redrawing the alignment interaction
This option allows one to specify how large the region surrounding the target site should be set when generating the alignment figure of the interaction
If you use this program in your work you might want to cite:
R. Lorenz, S.H. Bernhart, C. Hoener zu Siederdissen, H. Tafer, C. Flamm, P.F. Stadler and I.L. Hofacker (2011), "ViennaRNA Package 2.0", Algorithms for Molecular Biology: 6:26
I.L. Hofacker, W. Fontana, P.F. Stadler, S. Bonhoeffer, M. Tacker, P. Schuster (1994), "Fast Folding and Comparison of RNA Secondary Structures", Monatshefte f. Chemie: 125, pp 167-188
R. Lorenz, I.L. Hofacker, P.F. Stadler (2016), "RNA folding with hard and soft constraints", Algorithms for Molecular Biology 11:1 pp 1-13
The calculation of duplex structure is based on dynamic programming algorithm originally developed by Rehmsmeier and in parallel by Hofacker.
H. Tafer and I.L. Hofacker (2008), "RNAplex: a fast tool for RNA-RNA interaction search.", Bioinformatics: 24(22), pp 2657-2663
S. Bonhoeffer, J.S. McCaskill, P.F. Stadler, P. Schuster (1993), "RNA multi-structure landscapes", Euro Biophys J: 22, pp 13-24
The energy parameters are taken from:
D.H. Mathews, M.D. Disney, D. Matthew, J.L. Childs, S.J. Schroeder, J. Susan, M. Zuker, D.H. Turner (2004), "Incorporating chemical modification constraints into a dynamic programming algorithm for prediction of RNA secondary structure", Proc. Natl. Acad. Sci. USA: 101, pp 7287-7292
D.H Turner, D.H. Mathews (2009), "NNDB: The nearest neighbor parameter database for predicting stability of nucleic acid secondary structure", Nucleic Acids Research: 38, pp 280-282
Hakim Tafer, Ivo L. Hofacker
If in doubt our program is right, nature is at fault. Comments should be sent to firstname.lastname@example.org.